Plasmid

Part:BBa_K4184002:Design

Designed by: Benjamin Harder, Fabienne Spang   Group: iGEM22_TU_Braunschweig   (2022-10-04)


pSB1C3-FRET


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 941
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 941
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was difficult to exchange the linker that was present with the TEV protease interface, as the linker could not be too long. Otherwise, the FRET energy transfer would no longer have worked.


Source

The Plasmid excluding the recognition site and the His-tag is from Thorsten Seidel

References